Inhibitory Effects of Chloroform Extracts Derived from Corbicula fluminea on the Release of Pro-inflammatory Cytokines

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• 作者：Ching-Min Lin ; Yu-Ling Lin ; Nu-Man Tsai ; Hsin-Yi Wu ; Shu-Yi Ho ; Chia-Hung Chen ; Yen-Ku Liu ; Yi-Han Chiu ; Li-Ping Ho ; Ru-Ping Lee ; Kuang-Wen Liao
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：April 25, 2012
• 年：2012
• 卷：60
• 期：16
• 页码：4076-4082
• 全文大小：401K
• 年卷期：v.60,no.16(April 25, 2012)
• ISSN：1520-5118

文摘

Corbicula fluminea, the primary freshwater bivalve cultivated in Taiwan, was formerly used as a remedy for hepatitis. Recent reports indicate that C. fluminea has many bioactivities, but it remains unknown whether C. fluminea affects inflammation. This study explored the anti-inflammatory activity of C. fluminea. C. fluminea was first treated with chloroform to obtain clam chloroform extracts (CCEs). On the basis of the assay for the release of pro-inflammatory cytokines in vitro and in vivo, the results show that the CCEs significantly lowered the release of lipopolysaccharide (LPS)-induced pro-inflammatory cytokines. Additionally, the CCEs reduced LPS-induced organ damage. Real-time polymerase chain reaction analysis suggested that CCEs inhibit the LPS-induced mRNA expression of interleukin-1尾 and tumor necrosis factor-伪. Western blot analysis indicated that the CCEs increased expression of I魏B and attenuated the phosphorylation of I魏B. Gas chromatography鈥搈ass spectrometry suggests that phytosterols and fatty acids are responsible for the anti-inflammatory properties of CCEs. Taken together, CCEs have the potential to be developed as an anti-inflammatory functional food.

Keywords:

Freshwater clam; IL-1尾; inflammation; I魏B-伪; TNF-伪