Biomimetic Engineering of a Generic Cell-on-Membrane Architecture by Microfluidic Engraving for On-Chip Bioassays

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• 作者：Sang-Wook Lee ; Ji-Yoon Noh ; Seung Chul Park ; Jin-Ho Chung ; Byoungho Lee ; Sin-Doo Lee
• 刊名：Langmuir
• 出版年：2012
• 出版时间：May 22, 2012
• 年：2012
• 卷：28
• 期：20
• 页码：7585-7590
• 全文大小：373K
• 年卷期：v.28,no.20(May 22, 2012)
• ISSN：1520-5827

文摘

We develop a biomimetic cell-on-membrane architecture in close-volume format which allows the interfacial biocompatibility and the reagent delivery capability for on-chip bioassays. The key concept lies in the microfluidic engraving of lipid membranes together with biological cells on a supported substrate with topographic patterns. The simultaneous engraving process of a different class of fluids is promoted by the front propagation of an air鈥搘ater interface inside a flow-cell. This highly parallel, microfluidic cell-on-membrane approach opens a door to the natural biocompatibility in mimicking cellular stimuli-response behavior essential for diverse on-chip bioassays that can be precisely controlled in the spatial and temporal manner.