Rapid Transformation of Protein-Caged Nanomaterials into Microbubbles As Bimodal Imaging Agents

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• 作者：Cheng-An J. Lin ; Wen-Kai Chuang ; Zih-Yun Huang ; Shih-Tsung Kang ; Ching-Yi Chang ; Ching-Ta Chen ; Jhih-Liang Li ; Jimmy K. Li ; Hsueh-Hsiao Wang ; Fu-Chen Kung ; Ji-Lin Shen ; Wen-Hsiung Chan ; Chih-Kuang Yeh ; Hung-I Yeh ; Wen-Fu T. Lai ; Walter H. Chang
• 刊名：ACS Nano
• 出版年：2012
• 出版时间：June 26, 2012
• 年：2012
• 卷：6
• 期：6
• 页码：5111-5121
• 全文大小：751K
• 年卷期：v.6,no.6(June 26, 2012)
• ISSN：1936-086X

文摘

We present a general method for converting colloidal nanomaterials into microbubbles as ultrasound contrast agents. Protein-caged nanomaterials, made either by self-assembled nanoparticles' protein corona or by fluorescent gold nanoclusters, can be rapidly transformed into microbubbles via a sonochemical route, which promote disulfide cross-linking of cysteine residues between protein-caged nanomaterials and free albumin during acoustic cavitation. The proposed methods yielded microbubbles with multiple functions by adjusting the original nanoparticle/protein mixture. We also showed a new dual-modal imaging agent of fluorescent gold microbubbles in vitro and in vivo, which can hold many potential applications in medical diagnostics and therapy.

Keywords:

fluorescent gold nanoclusters; quantum dots; ultrasound; microbubbles; protein corona; bimodal imaging