Highly Sensitive Simultaneous Detection of Lead(II) and Barium(II) with G-Quadruplex DNA in 伪-Hemolysin Nanopore

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• 作者：Chun Yang ; Lei Liu ; Tao Zeng ; Daowu Yang ; Zhiyi Yao ; Yuliang Zhao ; Hai-Chen Wu
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：August 6, 2013
• 年：2013
• 卷：85
• 期：15
• 页码：7302-7307
• 全文大小：335K
• 年卷期：v.85,no.15(August 6, 2013)
• ISSN：1520-6882

文摘

Both Pb²⁺ and Ba²⁺ can bind with high affinity to some specific DNA sequences, inducing the formation of unimolecular G-quadruplex structures. Translocation of a DNA probe containing such sequences through an 伪-hemolysin nanopore in the presence of Pb²⁺ or Ba²⁺ would result in much prolonged DNA translocation events. Quantification of these events can reveal the concentrations of Pb²⁺ or Ba²⁺ at as low as 0.8 nM. Besides, Pb²⁺ and Ba²⁺ in the solution can be simultaneously detected and individually identified. Furthermore, the probe is highly selective for Pb²⁺ and Ba²⁺ detection without interference from other metal ions. This sensing strategy can be extended to many other analytes which can bind to DNA molecules with high affinity.