Quantitative Analysis of Axonal Transport by Using Compartmentalized and Surface Micropatterned Culture of Neurons

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• 作者：Hyung Joon Kim ; Jeong Won Park ; Jae Hwan Byun ; Wayne W. Poon ; Carl W. Cotman ; Charless C. Fowlkes ; Noo Li Jeon
• 刊名：ACS Chemical Neuroscience
• 出版年：2012
• 出版时间：June 20, 2012
• 年：2012
• 卷：3
• 期：6
• 页码：433-438
• 全文大小：327K
• 年卷期：v.3,no.6(June 20, 2012)
• ISSN：1948-7193

文摘

Mitochondria, synaptic vesicles, and other cytoplasmic constituents have to travel long distance along the axons from cell bodies to nerve terminals. Interruption of this axonal transport may contribute to many neurodegenerative diseases including Alzheimer鈥檚 disease (AD). It has been recently shown that exposure of cultured neurons to 尾-amyloid (A尾) resulted in severe impairment of mitochondrial transport. This Letter describes an integrated microfluidic platform that establishes surface patterned and compartmentalized culture of neurons for studying the effect of A尾 on mitochondria trafficking in full length of axons. We have successfully quantified the trafficking of fluorescently labeled mitochondria in distal and proximal axons using image processing. Selective treatment of A尾 in the somal or axonal compartments resulted in considerable decrease in mitochondria movement in a location dependent manner such that mitochondria trafficking slowed down more significantly proximal to the location of A尾 exposure. Furthermore, this result suggests a promising application of microfluidic technology for investigating the dysfunction of axonal transport related to neurodegenerative diseases.

Keywords:

尾-Amyloid; axonal transport; mitochondrial trafficking; microfludics; surface micropatterning; image processing