Discovery, Isolation, and Structure Elucidation of Dretamycin

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• 作者：Kenneth E. Wilson ; Scott K. Smith ; Rosemarie Kelly ; Prakash Masurekar ; Deming Xu ; Craig A. Parish ; Hao Wang ; Debbie L. Zink ; Julian E. Davies ; Terry Roemer
• 刊名：Journal of Natural Products
• 出版年：2014
• 出版时间：June 27, 2014
• 年：2014
• 卷：77
• 期：6
• 页码：1280-1286
• 全文大小：340K
• ISSN：1520-6025

文摘

The Candida albicans fitness test is a whole cell screening platform that utilizes a mixed-pool of C. albicans mutants, each of which carries a heterozygous deletion of a particular gene. In the presence of an antifungal inhibitor, a subset of these mutants exhibits a growth phenotype of hypersensitivity or hyposensitivity. Collectively these mutants reflect aspects of the mechanism of action of the compound in question. In the course of screening natural products a culture of Streptomyces sp. MS-1-4 was discovered to produce a compound, dretamycin, which yielded a fitness profile exhibiting significant hypersensitivity of the DRE2 heterozygote and hyposensitivity of the DIP5 heterozygote. Herein we report the production, isolation, and structure elucidation of dretamycin.