Toxicity Assessment of Transgenic Papaya Ringspot Virus of 823-2210 Line Papaya Fruits

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• 作者：Hsin-Tang Lin ; Gow-Chin Yen ; Ting-Tzu Huang ; Lit-Fu Chan ; Ying-Huey Cheng ; Jhaol-Huei Wu ; Shyi-Dong Yeh ; Sheng-Yang Wang ; Jiunn-Wang Liao
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：February 20, 2013
• 年：2013
• 卷：61
• 期：7
• 页码：1585-1596
• 全文大小：382K
• 年卷期：v.61,no.7(February 20, 2013)
• ISSN：1520-5118

文摘

The transgenic papaya is a valuable strategy for creating plants resistant to papaya ringspot virus (PRSV) infection and increasing production. This study was further performed to evaluate the comparative toxicity effects of the newly developed transgenic line of the fruits of two backcross transgenic papaya lines (2210 and 823) and one hybrid line (823-2210) and compare to their parent non-transgenic (TN-2) counterparts. The stability analysis of coat protein (CP) of PRSV was investigated using the digestion stability assays in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and bile salts to detect the CP fragments. Results revealed that the CP fragments were rapidly hydrolyzed in SGF and were undetectable in organs and gastrointestinal contents in rats. For the genotoxicity, three in vitro assays were conducted and exhibited that non-transgenic and backcross transgenic papaya fruits were negative. Moreover, a repeated animal feeding study was conducted by feeding 2 g/kg of body weight (bw) of non-transgenic and backcross transgenic papaya fruits for 28 days in rats. There were no biological or toxicological significances between non-transgenic and backcross transgenic papaya fruits in rats. The results demonstrated that the backcross transgenic papaya fruit can be recognized as an equivalent substitution for traditional papaya in food safety.

Keywords:

Transgenic papaya; stability analysis; genotoxicity; food safety