HMDO-Promoted Peptide and Protein Synthesis in Ionic Liquids

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• 作者：Jianli Duan ; Yao Sun ; Hao Chen ; Guofu Qiu ; Haibing Zhou ; Ting Tang ; Zixin Deng ; Xuechuan Hong
• 刊名：The Journal of Organic Chemistry
• 出版年：2013
• 出版时间：July 19, 2013
• 年：2013
• 卷：78
• 期：14
• 页码：7013-7022
• 全文大小：479K
• 年卷期：v.78,no.14(July 19, 2013)
• ISSN：1520-6904

文摘

Hexamethyldisiloxane (HMDO) has been developed to efficiently promote the metal-free direct coupling of an amino function of one cysteine-free peptide or protein and a C-terminal thioester of the second peptide in ionic liquids. The amide-coupling reaction proceeds smoothly under mild conditions to afford the corresponding products in good to excellent yields (63鈥?4%). Peptide couplings were also achieved using in-situ-generated thioesters by the thioesterification of oxo esters.