Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

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• 作者：Hsu-Hua Yeh ; Shu-Lin Chang ; Yi-Ming Chiang ; Kenneth S. Bruno ; Berl R. Oakley ; Tung-Kung Wu ; Clay C. C. Wang
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：February 15, 2013
• 年：2013
• 卷：15
• 期：4
• 页码：756-759
• 全文大小：234K
• 年卷期：v.15,no.4(February 15, 2013)
• ISSN：1523-7052

文摘

We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA螖R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.