Highly Efficient Preparation of Multiscaled Quantum Dot Barcodes for Multiplexed Hepatitis B Detection

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• 作者：Gang Wang ; Yuankui Leng ; Hongjing Dou ; Lu Wang ; Wanwan Li ; Xiebing Wang ; Kang Sun ; Lisong Shen ; Xiangliang Yuan ; Jiyu Li ; Kun Sun ; Junsong Han ; Huasheng Xiao ; Yue Li
• 刊名：ACS Nano
• 出版年：2013
• 出版时间：January 22, 2013
• 年：2013
• 卷：7
• 期：1
• 页码：471-481
• 全文大小：843K
• 年卷期：v.7,no.1(January 22, 2013)
• ISSN：1936-086X

文摘

Both disease diagnosis and therapeutic treatments require real-time information from assays capable of identifying multiple targets. Among various multiplexed biochips, multiplexed suspension assays of quantum dot (QD)-encoded microspheres are highly advantageous. This arises from the excellent fluorescent properties of the QDs incorporated into these microspheres, thus allowing them to serve as 鈥淨D barcodes鈥? QD barcodes can be prepared through various approaches. However, the formulation of improved synthetic techniques that may allow more efficient preparation of QD barcodes with better encoding accuracy still remains a challenge. In this report, we describe a combined membrane emulsification鈥搒olvent evaporation (MESE) approach for the efficient preparation of QD barcodes. By combining the advantages of the MESE approach in controlling the barcode sizes with accurate encoding, a three-dimensional barcode library that integrates the signals of the forward scattering, fluorescence 1, and fluorescence 4 channels was established via flow cytometry. The five indexes of hepatitis B viruses were chosen as diagnostic targets to examine the feasibility of the QD barcodes in high-throughput diagnosis. On the basis of showing that singleplex detection is feasible, we demonstrate the ability of these QD barcodes to simultaneously and selectively detect a multitude of diverse biomolecular targets.

Keywords:

quantum dots; microspheres; Shirasu porous glass membrane emulsification; flow cytometry; suspension immunoassay