NaGdF4 Nanoparticle-Based Molecular Probes for Magnetic Resonance Imaging of Intraperitoneal Tumor Xenografts in Vivo

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• 作者：Yi Hou ; Ruirui Qiao ; Fang Fang ; Xuxia Wang ; Chengyan Dong ; Kan Liu ; Chunyan Liu ; Zhaofei Liu ; Hao Lei ; Fan Wang ; Mingyuan Gao
• 刊名：ACS Nano
• 出版年：2013
• 出版时间：January 22, 2013
• 年：2013
• 卷：7
• 期：1
• 页码：330-338
• 全文大小：542K
• 年卷期：v.7,no.1(January 22, 2013)
• ISSN：1936-086X

文摘

Differently sized NaGdF₄ nanocrystals with narrow particle size distributions were synthesized by a high temperature approach. Upon ligand exchange, the as-prepared hydrophobic NaGdF₄ nanocrystals were transferred into water by using asymmetric PEGs simultaneously bearing phosphate and maleimide groups. Further investigations demonstrated that the water-soluble NaGdF₄ nanocrystals, coated by PEG bearing two phosphate groups on the same side, exhibit not only excellent colloidal stability in water and PBS buffer, but also higher T1 relaxivity than Gd-DTPA (Magnevist). Through 鈥渃lick鈥?reaction between the maleimide residue on particle surface and thiol group from the partly reduced anti-EGFR monoclonal antibody (mAb), NaGdF₄鈥揚EG-mAb nanoprobes were constructed, and their biocompatibility and binding specificity were evaluated through in vitro experiments. A series of in vivo experiments were then carried out for detecting intraperitoneal tumor xenografts in nude mice by using magnetic resonance (MR) imaging technique. The results revealed that the NaGdF₄鈥揚EG-mAb probes possessed satisfying tumor-specific targeting ability and strong MR contrast enhancement effects.

Keywords:

NaGdF₄ nanoparticles; ligand exchange; T1 contrast agent; MR molecular imaging probe; intraperitoneal tumor xenografts