Dissect Conformational Distribution and Drug-Induced Population Shift of Prokaryotic rRNA A-Site

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• 作者：Jia Lu ; Liang Zhao ; Amy Xia ; Tianbing Xia ; Xin Qi
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：March 12, 2013
• 年：2013
• 卷：52
• 期：10
• 页码：1651-1653
• 全文大小：209K
• 年卷期：v.52,no.10(March 12, 2013)
• ISSN：1520-4995

文摘

The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a drug-specific manner. Motions of bases on nanosecond and picosecond time scales are differentially affected by the drug binding. Our results underscore the importance of understanding the detailed dynamic picture of molecular recognition by resolving dynamics in the distinct picosecond time regime and facilitate development of antimicrobial drugs targeting dynamic RNAs.