Surface-Enhanced Raman Scattering-Based Sensing In Vitro: Facile and Label-Free Detection of Apoptotic Cells at the Single-Cell Level

详细信息    查看全文

• 作者：Xiangxu Jiang ; Ziyun Jiang ; Tingting Xu ; Shao Su ; Yiling Zhong ; Fei Peng ; Yuanyuan Su ; Yao He
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：March 5, 2013
• 年：2013
• 卷：85
• 期：5
• 页码：2809-2816
• 全文大小：604K
• 年卷期：v.85,no.5(March 5, 2013)
• ISSN：1520-6882

文摘

Surface-enhanced Raman scattering (SERS) is well recognized as a powerful analytical tool, enabling ultrahigh sensitive detection of analytes at low concentrations, even down to single-molecule level. Of particular note, in comparison to sufficient investigations on SERS-based detection of biomolecules (e.g., DNA and protein), there has been relatively scanty information regarding in vitro and in vivo detection. In this Article, we demonstrate a kind of SERS-active substrate, i.e., AgNPs-decorated silicon wafer (AgNPs@Si), as a high-performance in vitro sensing platform for single-cell detection of apoptotic cells. The AgNPs@Si yields highly reproducible SERS signals with an enhancement factor of 10⁷. Remarkably, cellular experiments show that facile, noninvasive, label-free, and sensitive detection of apoptotic cells is readily realized using the high-performance SERS-active platform. Three kinds of apoptotic cells treated with apoptosis inducer are facilely and sensitively detected at the single-cell level, suggesting the exciting potential of AgNPs@Si for SERS-based in vitro analysis and detection.