Glycoform Remodeling Generates a Synthetic T Cell Phenotype

详细信息    查看全文

• 作者：Chunxia Zou ; Ravi S. Loka ; Yi Zhang ; Christopher W. Cairo
• 刊名：Bioconjugate Chemistry
• 出版年：2013
• 出版时间：June 19, 2013
• 年：2013
• 卷：24
• 期：6
• 页码：907-914
• 全文大小：352K
• 年卷期：v.24,no.6(June 19, 2013)
• ISSN：1520-4812

文摘

The glycan of specific proteins can dictate the response of cells to stimuli, and thus their phenotype. We describe a chemical strategy to modify the cellular glycoform of T cells, which resulted in a modified cellular response. Our data indicate that chemical modification of the phosphatase CD45 is responsible for the observed differences in response to receptor cross-linking. By increasing the content of galactose epitopes in the glycocalyx of a lymphoma cell line, we were able to increase the response of the cell to lectin stimulation through the glycoprotein receptor, CD45. The method described here exploits metabolic labeling of a cell to reprogram the cellular response to external stimuli though changes in the number of lectin binding sites on the cell surface.