Organic Silicone Sol鈥揋el Polymer as a Noncovalent Carrier of Receptor Proteins for Label-Free Optical Biosensor Application

详细信息    查看全文

• 作者：Jun Ren ; Linghua Wang ; Xiuyou Han ; Jianfang Cheng ; Huanlin Lv ; Jinyan Wang ; Xigao Jian ; Mingshan Zhao ; Lingyun Jia
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：January 23, 2013
• 年：2013
• 卷：5
• 期：2
• 页码：386-394
• 全文大小：421K
• 年卷期：v.5,no.2(January 23, 2013)
• ISSN：1944-8252

文摘

Optical biosensing techniques have become of key importance for label-free monitoring of biomolecular interactions in the current proteomics era. Together with an increasing emphasis on high-throughput applications in functional proteomics and drug discovery, there has been demand for facile and generally applicable methods for the immobilization of a wide range of receptor proteins. Here, we developed a polymer platform for microring resonator biosensors, which allows the immobilization of receptor proteins on the surface of waveguide directly without any additional modification. A sol鈥揼el process based on a mixture of three precursors was employed to prepare a liquid hybrid polysiloxane, which was photopatternable for the photocuring process and UV imprint. Waveguide films were prepared on silicon substrates by spin coating and characterized by atomic force microscopy for roughness, and protein adsorption. The results showed that the surface of the polymer film was smooth (rms = 0.658 nm), and exhibited a moderate hydrophobicity with the water contact angle of 97掳. Such a hydrophobic extent could provide a necessary binding strength for stable immobilization of proteins on the material surface in various sensing conditions. Biological activity of the immobilized Staphylococcal protein A and its corresponding biosensing performance were demonstrated by its specific recognition of human Immunoglobulin G. This study showed the potential of preparing dense, homogeneous, specific, and stable biosensing surfaces by immobilizing receptor proteins on polymer-based optical devices through the direct physical adsorption method. We expect that such polymer waveguide could be of special interest in developing low-cost and robust optical biosensing platform for multidimensional arrays.

Keywords:

optical biosensor; microring resonators; sol鈭抔el polymer; surface modification; protein immobilization; physical adsorption