A DNAzyme-Gold Nanoparticle Probe for Uranyl Ion in Living Cells

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• 作者：Peiwen Wu ; Kevin Hwang ; Tian Lan ; Yi Lu
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：April 10, 2013
• 年：2013
• 卷：135
• 期：14
• 页码：5254-5257
• 全文大小：260K
• 年卷期：v.135,no.14(April 10, 2013)
• ISSN：1520-5126

文摘

DNAzymes have shown great promise as a general platform for detecting metal ions, as many metal-specific DNAzymes can be obtained using in vitro selection. While DNAzyme-based metal sensors have found many applications in the extracellular environment, no intracellular application of DNAzyme sensors has yet been reported. Here, we demonstrate a novel type of metal ion sensor for intracellular metal ion detection. The probe consists of a 13 nm gold nanoparticle (AuNP) core functionalized with a shell consisting of a uranyl-specific 39E DNAzyme whose enzyme strand contains a thiol at the 3鈥?end for conjugation to the AuNP, and whose substrate strand is modified with a Cy3 fluorophore at the 5鈥?end and a molecular quencher at the 3鈥?end. In the absence of uranyl, the fluorescence of the Cy3 is quenched by both AuNP and the molecular quencher. In the presence of uranyl, the DNAzyme cleaves the fluorophore-labeled substrate strand, resulting in release of the shorter product strand containing the Cy3 and increased fluorescence. We demonstrate that this DNAzyme-AuNP probe can readily enter cells and can serve as a metal ion sensor within a cellular environment, making it the first demonstration of DNAzymes as intracellular metal ion sensors. Such a method can be generally applied to the detection of other metal ions using other DNAzymes selected through in vitro selection.