Enzyme Immobilization on Carboxyl-Functionalized Graphene Oxide for Catalysis in Organic Solvent

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• 作者：Qingzhong Li ; Fei Fan ; Yang Wang ; Wei Feng ; Peijun Ji
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：May 15, 2013
• 年：2013
• 卷：52
• 期：19
• 页码：6343-6348
• 全文大小：326K
• 年卷期：v.52,no.19(May 15, 2013)
• ISSN：1520-5045

文摘

Carboxyl-functionalized graphene oxide (GO鈥揅OOH) was utilized to immobilize lipase. Fourier transform infrared (FTIR), UV鈥搗isible, and X-ray photoelectron (XPS) spectra were measured to characterize lipase immobilization. At the optimal temperature of 40 掳C, the immobilized lipase retains 80% of the hydrolytic activity of the native lipase. For catalyzing the enantioselective reaction in the organic solvent heptane, at 50 掳C (optimal), the catalysis efficiency of the immobilized lipase is 1.6 times that of native lipase, and the immobilized lipase retains the selectivity of the native lipase. This work demonstrates that graphene oxide is a suitable support for immobilization of lipase for catalysis in organic solvent.