RCA Strands as Scaffolds To Create Nanoscale Shapes by a Few Staple Strands

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• 作者：Yinzhou Ma ; Hongning Zheng ; Cuie Wang ; Qin Yan ; Jie Chao ; Chunhai Fan ; Shou-Jun Xiao
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：February 27, 2013
• 年：2013
• 卷：135
• 期：8
• 页码：2959-2962
• 全文大小：391K
• 年卷期：v.135,no.8(February 27, 2013)
• ISSN：1520-5126

文摘

Thousands of nucleotide(nt)-long single strand DNAs, generated from rolling-circle-amplification (RCA), were used as scaffolds to create DNA nanoscale wires and plates with a few short staple strands by following the origami design principle with a crossover at 1.5 turns. The core sequence of the circle template, for producing tens and hundreds of tandemly repeated copies of it by RCA, was designed according to Seeman鈥檚 sequence design principle for nucleic acid structural engineering (Seeman, N. C. J. Biomol. Struct. Dyn. 1990, 8, 573). The significance for folding the RCA products into nanoscale shapes lies in the design flexibility of both staple and scaffold strand codes, simplicity of a few short staple strands to fold the periodic sequence of RCA products, and lower cost.