Simultaneous Determination of Banned Acid Orange Dyes and Basic Orange Dyes in Foodstuffs by Liquid Chromatography鈥揟andem Electrospray Ionization Mass Spectrometry via Negative/Positive Ion Switching

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• 作者：Guozhen Fang ; Yu Wu ; Xiaomeng Dong ; Cuicui Liu ; Shaoyuan He ; Shuo Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：April 24, 2013
• 年：2013
• 卷：61
• 期：16
• 页码：3834-3841
• 全文大小：430K
• 年卷期：v.61,no.16(April 24, 2013)
• ISSN：1520-5118

文摘

Simultaneous detection of two classes of dyes possessing different chemical properties is difficult. In this study, through negative/positive ion switching mode, simultaneous determination of four typical acid orange dyes and three typical basic orange dyes was achieved by a single high-performance liquid chromatography鈥搕andem mass spectrometry (LC-MS/MS) method, and the analytical efficiency of multiresidues identification was greatly improved. To enhance detection sensitivity, the sample pretreatment conditions and HPLC-MS/MS determining conditions were carefully optimized. Under optimal conditions, good linearity was obtained over the range of 5鈥?00 渭g L^鈥? with a correlation coefficient (R²) >0.9998. Limits of detection (LODs) and limits of quantification (LOQs) of the seven dyes were 0.5鈥?.0 and 2.0鈥?.0 渭g kg^鈥?, respectively. The recoveries of the seven dyes in soybean products and marinated eggs were 74鈥?26% with relative standard deviations (RSDs) of 2.22鈥?5.4%, suggesting the developed method is promising for the accurate quantification of the seven dyes at trace levels in foods.

Keywords:

acid orange dyes; basic orange dyes; foodstuffs; LC-MS/MS; negative/positive ion switching mode