Eudesmanolides from Wedelia trilobata (L.) Hitchc. as Potential Inducers of Plant Systemic Acquired Resistance

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• 作者：Yating Li ; Xiaojiang Hao ; Shifei Li ; Hongping He ; Xiaohui Yan ; Yongdui Chen ; Jiahong Dong ; Zhongkai Zhang ; Shunlin Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：April 24, 2013
• 年：2013
• 卷：61
• 期：16
• 页码：3884-3890
• 全文大小：305K
• 年卷期：v.61,no.16(April 24, 2013)
• ISSN：1520-5118

文摘

Ten eudesmanolides (1鈥?b>10) including five new ones (1鈥?b>4 and 6) were isolated from the whole plant of Wedelia trilobata (L.) Hitchc., a notoriously invasive weed in South China. As main constituents of W. trilobata, eight of these eudesmanolides were tested for their antitobacco mosaic virus (TMV) activities by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the tested compounds, at 10 渭g/mL, showed strong antiviral activities in the pretreated tobacco plants with inhibition rates ranging from 46.7% to 76.5%, significantly higher than that of the positive control, ningnanmycin (13.5%). Their potential of inducing systemic acquired resistance (SAR) was also evaluated, and compounds 1 and 8 showed excellent induction activities. Furthermore, it was found that different concentrations of compound 1 promoted phenylalanine ammonia-lyase (PAL) activity in tobacco plants. To our knowledge, this is the first report that eudesmanolides could induce resistance in tobacco plants against the viral pathogen TMV.

Keywords:

eudesmanolides; Wedelia trilobata (L.) Hitchc.; tobacco mosaic virus; systemic acquired resistance