Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)

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• 作者：Linn Fagerberg ; Per Oksvold ; Marie Skogs ; Cajsa 脛lgen盲s ; Emma Lundberg ; Fredrik Pont茅n ; 脜sa Sivertsson ; Jacob Odeberg ; Daniel Klevebring ; Caroline Kampf ; Anna Asplund ; Evelina Sj枚stedt ; Cristina Al-Khalili Szigyarto ; Per-Henrik Edqvist ; IngMarie Olsson ; Urban Rydberg ; Paul Hudson ; Jenny Ottosson Takanen ; Holger Berling ; Lisa Bj枚rling ; Hanna Tegel ; Johan Rockberg ; Peter Nilsson ; Sanjay Navani ; Karin Jirstr枚m ; Jan Mulder ; Jochen M. Schwenk ; Martin Zwahlen ; Sophia Hober ; Mattias Forsberg ; Kalle von Feilitzen ; Mathias Uhl茅n
• 刊名：Journal of Proteome Research
• 出版年：2013
• 出版时间：June 7, 2013
• 年：2013
• 卷：12
• 期：6
• 页码：2439-2448
• 全文大小：637K
• 年卷期：v.12,no.6(June 7, 2013)
• ISSN：1535-3907

文摘

A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approximately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).

Keywords:

Antibody-based protein profiling; C-HPP