Dual Molecular Recognition Leading to a Protein鈥揚olymer Conjugate and Further Self-Assembly

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• 作者：Kongchang Wei ; Jun Li ; Guosong Chen ; Ming Jiang
• 刊名：ACS Macro Letters
• 出版年：2013
• 出版时间：March 19, 2013
• 年：2013
• 卷：2
• 期：3
• 页码：278-283
• 全文大小：323K
• 年卷期：v.2,no.3(March 19, 2013)
• ISSN：2161-1653

文摘

Supramolecular conjugation between native protein concanavalin A (ConA) and synthetic polymer PEG (polyethylene glycol) was achieved by dual molecular recognition interactions via a linker, 尾CD-Man, of which 尾-cyclodextrin (尾CD) and 伪-mannopyranoside (Man) recognized the adamantane (Ada) end of PEG and lectin ConA orthogonally. Further self-assembly of the resultant supra-conjugates of ConA-PEG was induced by the addition of 伪CD, which was selectively threaded by PEG chains, leading to nanoparticles in dilute solution or hydrogel at a higher concentration. The moduli of the obtained hydrogel were three magnitudes higher than those of the control sample without ConA, showing the dramatic cross-linking effect of ConA achieved by its rather weak interaction with 伪-d-mannopyranoside.