Enzymatic Stability and Immunoregulatory Efficacy of a Synthetic Indolicidin Analogue with Regular Enantiomeric Sequence

详细信息    查看全文

• 作者：Ching-Yun Chang ; Chih-Wei Lin ; Sheng-Kuo Chiang ; Po-Ling Chen ; Chiung-Yi Huang ; Shih-Jen Liu ; Pele Chong ; Ming-Hsi Huang
• 刊名：ACS Medicinal Chemistry Letters
• 出版年：2013
• 出版时间：June 13, 2013
• 年：2013
• 卷：4
• 期：6
• 页码：522-526
• 全文大小：441K
• 年卷期：v.4,no.6(June 13, 2013)
• ISSN：1948-5875

文摘

Cell-mediated immunity plays a major role in protecting the host from viral infections and tumor challenge. Here, we report the enzymatic stability and adjuvanticity of a peptiomimetic stereoisomer of the bovine neutrophil peptide indolicidin. The analogue, dubbed ld-indolicidin, contains the regular enantiomeric sequence of indolicidin and is synthesized by general stepwise solid-phase strategy. ld-Indolicidin possesses high resistance to enzymatic degradation and shows tolerance in mice. As vaccine adjuvant, ld-indolicidin is better able than the native form of indolicidin to enhance cell-mediated immune responses, using inactivated H5N1 virus as a model antigen. Taken together, these results open up a new approach to the development of vaccine adjuvants and immunotherapy technologies.

Keywords:

Host defense peptides; immunoregulation; peptidomimetic stereoisomer; vaccine adjuvants