DNA Manipulation and Separation in Sublithographic-Scale Nanowire Array

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• 作者：Takao Yasui ; Sakon Rahong ; Koki Motoyama ; Takeshi Yanagida ; Qiong Wu ; Noritada Kaji ; Masaki Kanai ; Kentaro Doi ; Kazuki Nagashima ; Manabu Tokeshi ; Masateru Taniguchi ; Satoyuki Kawano ; Tomoji Kawai ; Yoshinobu Baba
• 刊名：ACS Nano
• 出版年：2013
• 出版时间：April 23, 2013
• 年：2013
• 卷：7
• 期：4
• 页码：3029-3035
• 全文大小：393K
• 年卷期：v.7,no.4(April 23, 2013)
• ISSN：1936-086X

文摘

Electrokinetic manipulations of biomolecules using artificial nanostructures within microchannels have proven capability for controlling the dynamics of biomolecules. Because there is an inherent spatial size limitation to lithographic technology, especially for nanostructures with a small diameter and high aspect ratio, manipulating a single small biomolecule such as in DNA elongation before nanopore sequencing is still troublesome. Here we show the feasibility for self-assembly of a nanowire array embedded in a microchannel on a fused silica substrate as a means to manipulate the dynamics of a single long T4-DNA molecule and also separate DNA molecules. High-resolution optical microscopy measurements are used to clarify the presence of fully elongated T4-DNA molecules in the nanowire array. The spatial controllability of sublithographic-scale nanowires within microchannels offers a flexible platform not only for manipulating and separating long DNA molecules but also for integrating with other nanostructures to detect biomolecules in methods such as nanopore sequencing.

Keywords:

nanowire array; nanobiodevices; electrokinetic manipulations; DNA dynamics; DNA separation