Spatially-Resolved EELS Analysis of Antibody Distribution on Biofunctionalized Magnetic Nanoparticles

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• 作者：Raul Arenal ; Laura De Matteis ; Laura Custardoy ; Alvaro Mayoral ; Marcel Tence ; Valeria Grazu ; Jes煤s M. De La Fuente ; Clara Marquina ; M. Ricardo Ibarra
• 刊名：ACS Nano
• 出版年：2013
• 出版时间：May 28, 2013
• 年：2013
• 卷：7
• 期：5
• 页码：4006-4013
• 全文大小：528K
• 年卷期：v.7,no.5(May 28, 2013)
• ISSN：1936-086X

文摘

Spatially resolved electron energy loss spectroscopy (SR-EELS) using scanning transmission electron microscope (STEM) allows the identification and determination of the spatial distribution of the components/elements of immuno-functionalized core鈥搒hell superparamagnetic magnetite nanoparticles. Here, we report that SR-EELS measurements allow the direct identification and study of the biological moieties (protein G and anti-HRP antibody) in complex bionanocarriers of relevance for biomedical applications. Our findings show that the biomacromolecules are located on specific areas on the nanoparticles鈥?surface. In addition, efficiency of this functionalization was evaluated by means of biochemical techniques.

Keywords:

EELS-STEM; antibody functionalization; organic/inorganic systems; magnetic nanoparticles