Carbon and Electron Fluxes during the Electricity Driven 1,3-Propanediol Biosynthesis from Glycerol

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• 作者：Mi Zhou ; Jingwen Chen ; Stefano Freguia ; Korneel Rabaey ; J眉rg Keller
• 刊名：Environmental Science & Technology
• 出版年：2013
• 出版时间：October 1, 2013
• 年：2013
• 卷：47
• 期：19
• 页码：11199-11205
• 全文大小：372K
• 年卷期：v.47,no.19(October 1, 2013)
• ISSN：1520-5851

文摘

1,3-Propanediol (1,3-PDO) can be produced biologically through glycerol fermentation. While such a process typically involves a pure culture system, particularly for crude glycerol, there would be operational advantages if a mixed population could be used. However, in the latter case the yield is typically low. Here, we use electrical current as the driving force for a mixed population fermenting glycerol in the cathode of a microbial bioelectrochemical system (BES). The carbon and electron flows were monitored by a titration and off-gas analysis (TOGA) sensor, and the syntrophic interactions in the BES were also investigated. Results show that on a carbon yield basis, current enhanced 1,3-PDO production from 24.8% (without current) to 50.1% (with a polarized biocathode at 鈭?.9 V versus standard hydrogen electrode, SHE). Flux analysis indicated that the reductive current can be integrated into glycerol metabolism to enhance 1,3-PDO yield and that glycerol metabolism was redirected from propionate fermentation to 1,3-PDO production. A polarization of 鈭?.6 V (vs SHE) resulted in more fermentative hydrogen production (from 2.7% to 8.0% on electron basis). 1,3-PDO production was also enhanced with hydrogen supply (37.7% on carbon basis), by suppressing hydrogen fermentation. Moreover, interspecies hydrogen transfer encouraged hydrogenotrophic methanogenesis, which was also accelerated by the cathodic polarization.