Dynamic and Reversible Fluorescence Imaging of Superoxide Anion Fluctuations in Live Cells and in Vivo

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• 作者：Wen Zhang ; Ping Li ; Fan Yang ; Xiufen Hu ; Chuanzhi Sun ; Wei Zhang ; Dezhan Chen ; Bo Tang
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：October 9, 2013
• 年：2013
• 卷：135
• 期：40
• 页码：14956-14959
• 全文大小：269K
• 年卷期：v.135,no.40(October 9, 2013)
• ISSN：1520-5126

文摘

Overgeneration of reactive oxygen species (ROS) is closely associated with cellular damage and diseases. As superoxide anion (O₂^{鈥⑩€?/sup>) is the precursor of other ROS, exploring O₂鈥⑩€?/sup> fluctuations in cells and in vivo is of great significance. To address this critical need, we have developed a novel reversible fluorescent probe with one-photon and two-photon fluorescence properties, which is well suited for monitoring O₂鈥⑩€?/sup> fluxes selectively and dynamically. Imaging results substantiate dynamic and reversible fluorescence responses of this probe to intracellular O₂鈥⑩€?/sup> under apoptotic stimuli. Moreover, this probe can conveniently visualize changes in O₂鈥⑩€?/sup> concentration during reperfusion injury in hepatocytes, zebrafish, and mice, by means of one-photon or two-photon imaging according to depths of various samples. The present study provides a powerful fluorescent imaging tool for dynamic tracking of O₂鈥⑩€?/sup> in live cells and in vivo.}