An Ultrasensitive Nanowire-Transistor Biosensor for Detecting Dopamine Release from Living PC12 Cells under Hypoxic Stimulation

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• 作者：Bor-Ran Li ; Ying-Jhu Hsieh ; Yan-Xi Chen ; Ya-Ting Chung ; Chien-Yuan Pan ; Yit-Tsong Chen
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：October 30, 2013
• 年：2013
• 卷：135
• 期：43
• 页码：16034-16037
• 全文大小：271K
• 年卷期：v.135,no.43(October 30, 2013)
• ISSN：1520-5126

文摘

Dopamine (DA) is an important neurotransmitter that is involved in neuronal signal transduction and several critical illnesses. However, the concentration of DA is extremely low in patients and is difficult to detect using existing electrochemical biosensors with detection limits typically around nanomolar levels (10^鈥? M). Here, we developed a nanoelectronic device as a biosensor for ultrasensitive and selective DA detection by modifying DNA-aptamers on a multiple-parallel-connected (MPC) silicon nanowire field-effect transistor (referred to as MPC aptamer/SiNW-FET). Compared with conventional electrochemical methods, the MPC aptamer/SiNW-FET has been demonstrated to improve the limit of DA detection to <10^鈥?1 M and to possess a detection specificity that is able to distinguish DA from other chemical analogues, such as ascorbic acid, catechol, phenethylamine, tyrosine, epinephrine, and norepinephrine. This MPC aptamer/SiNW-FET was also applied to monitor DA release under hypoxic stimulation from living PC12 cells. The real-time recording of the exocytotic DA induced by hypoxia reveals that the increase in intracellular Ca²⁺ that is required to trigger DA secretion is dominated by an extracellular Ca²⁺ influx, rather than the release of intracellular Ca²⁺ stores.