High Transfection Efficiency of Homogeneous DNA Nanoparticles Induced by Imidazolium Gemini Surfactant as Nonviral Vector

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• 作者：Ting Zhou ; Axel Llizo ; Ping Li ; Chenxuan Wang ; Yuanyuan Guo ; Mingqi Ao ; Linling Bai ; Chen Wang ; Yanlian Yang ; Guiying Xu
• 刊名：Journal of Physical Chemistry C
• 出版年：2013
• 出版时间：December 19, 2013
• 年：2013
• 卷：117
• 期：50
• 页码：26573-26581
• 全文大小：452K
• 年卷期：v.117,no.50(December 19, 2013)
• ISSN：1932-7455

文摘

Nonviral vectors are highly desirable for the development of efficient gene delivery systems. In this study, we report the monomolecular condensation of plasmid DNA and efficient cell transfection by imidazolium gemini surfactants ([C₁₂-4-C₁₂im]Br₂), which could be a potential nonviral vector for efficient gene therapy. Homogeneous DNA/[C₁₂-4-C₁₂im]Br₂ nanoparticles are formed with a diameter of approximately 100 nm and investigated by using atomic force microscopy. DNA condensates evolve from supercoiled DNA molecules, to individual toroids, to close-packed particles, and eventually to multimolecular aggregates with the increase of [C₁₂-4-C₁₂im]Br₂ concentrations. Highly efficient gene transfection in vitro is demonstrated in human embryonic kidney 293 (HEK293) and HeLa cells, which could be attributed to the effective DNA condensation into uniform nanoparticles induced by [C₁₂-4-C₁₂im]Br₂. In addition, the low cytotoxicity of [C₁₂-4-C₁₂im]Br₂ at transfection concentration region verified by cell viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, MTT assay) also supports [C₁₂-4-C₁₂im]Br₂ as an effective gene vector. The high gene transfection efficiency by [C₁₂-4-C₁₂im]Br₂ as well as its low cytotoxicity could shed light on the rational molecular design of nonviral vectors for gene delivery systems.