Lipo-chitooligosaccharidic Symbiotic Signals Are Recognized by LysM Receptor-Like Kinase LYR3 in the Legume Medicago truncatula

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• 作者：Judith Fliegmann ; Sophie Canova ; Christophe Lachaud ; Sandra Uhlenbroich ; Virginie Gasciolli ; Carole Pichereaux ; Michel Rossignol ; Charles Rosenberg ; Marie Cumener ; Delphine Pitorre ; Benoit Lefebvre ; Clare Gough ; Eric Samain ; S茅bastien Fort ; Hugues Driguez ; Boris Vauzeilles ; Jean-Marie Beau ; Alessandra Nurisso ; Anne Imberty ; Julie Cullimore ; Jean-Jacques Bono
• 刊名：ACS Chemical Biology
• 出版年：2013
• 出版时间：September 20, 2013
• 年：2013
• 卷：8
• 期：9
• 页码：1900-1906
• 全文大小：451K
• 年卷期：v.8,no.9(September 20, 2013)
• ISSN：1554-8937

文摘

While chitooligosaccharides (COs) derived from fungal chitin are potent elicitors of defense reactions, structurally related signals produced by certain bacteria and fungi, called lipo-chitooligosaccharides (LCOs), play important roles in the establishment of symbioses with plants. Understanding how plants distinguish between friend and foe through the perception of these signals is a major challenge. We report the synthesis of a range of COs and LCOs, including photoactivatable probes, to characterize a membrane protein from the legume Medicago truncatula. By coupling photoaffinity labeling experiments with proteomics and transcriptomics, we identified the likely LCO-binding protein as LYR3, a lysin motif receptor-like kinase (LysM-RLK). LYR3, expressed heterologously, exhibits high-affinity binding to LCOs but not COs. Homology modeling, based on the Arabidopsis CO-binding LysM-RLK AtCERK1, suggests that LYR3 could accommodate the LCO in a conserved binding site. The identification of LYR3 opens up ways for the molecular characterization of LCO/CO discrimination.