Lateral Flow Immunochromatographic Assay for Sensitive Pesticide Detection by Using Fe3O4 Nanoparticle Aggregates as Color Reagents

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• 作者：Chunyan Liu ; Qiaojuan Jia ; Chunhui Yang ; Ruirui Qiao ; Lihong Jing ; Libing Wang ; Chuanlai Xu ; Mingyuan Gao
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：September 1, 2011
• 年：2011
• 卷：83
• 期：17
• 页码：6778-6784
• 全文大小：907K
• 年卷期：v.83,no.17(September 1, 2011)
• ISSN：1520-6882

文摘

Magnetic Fe₃O₄ particle aggregates were prepared by cross-linking Fe₃O₄ nanoparticles bearing surface carbonyl groups with poly-l-lysine. Upon further coupling with antiparaoxon methyl polyclonal antibody, the resultant particle aggregate-based probes were used in a lateral flow immunochromatographic assay (LFIA) of pesticide residue of paraoxon methyl. The results were compared with that achieved by using the mother Fe₃O₄ nanoparticles. More quantitative results on the signal amplification effect endowed by the controlled aggregation of Fe₃O₄ nanoparticles were extracted by relative optical density analysis. Under optimized conditions, a detection limit of 1.7 ng/mL for paraoxon methyl was achieved by using the particle aggregates, which is almost 40-fold lower than that based on the mother Fe₃O₄ nanoparticles.