Gadolinium Oxide Nanoparticles and Aptamer-Functionalized Silver Nanoclusters-Based Multimodal Molecular Imaging Nanoprobe for Optical/Magnetic Resonance Cancer Cell Imaging

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• 作者：Jingjing Li ; Jia You ; Yue Dai ; Meilin Shi ; Cuiping Han ; Kai Xu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：November 18, 2014
• 年：2014
• 卷：86
• 期：22
• 页码：11306-11311
• 全文大小：361K
• ISSN：1520-6882

文摘

Multimodal molecular imaging has attracted more and more interest from researchers due to its combination of the strengths of each imaging modality. The development of specific and multifunctional molecular imaging probes is the key for this method. In this study, we fabricated an optical/magnetic resonance (MR) dual-modality molecular imaging nanoprobe, polyethylene glycol-coated ultrasmall gadolinium oxide (PEG-Gd₂O₃)/aptamer-Ag nanoclusters (NCs), for tracking cancer cells. To achieve this aim, PEG-Gd₂O₃ nanoparticles (NPs) as magnetic resonance imaging (MRI) contrast agent and aptamer functionalized silver nanoclusters (aptamer-Ag NCs) as fluorescence reporter were first synthesized by a one-pot approach, respectively. They were then conjugated by the covalent coupling reaction between the carboxyl group on the surface of PEG-Gd₂O₃ NPs and amino group modified on the 5鈥?end of AS1411 aptamer. With a suitable ratio, the fluorescence intensity of aptamer-Ag NCs and MR signal of PEG-Gd₂O₃ nanoparticles could both be enhanced after the formation of PEG-Gd₂O₃/aptamer-Ag NCs nanoprobe, which favored their application for multimodal molecular imaging. With this nanoprobe, MCF-7 tumor cells could be specifically tracked by both fluorescence imaging and magnetic resonance imaging in vitro.