Heterogeneous Nano Metal鈥揙rganic Framework Fluorescence Probe for Highly Selective and Sensitive Detection of Hydrogen Sulfide in Living Cells

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• 作者：Yu Ma ; Hao Su ; Xuan Kuang ; Xiangyuan Li ; Tingting Zhang ; Bo Tang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：November 18, 2014
• 年：2014
• 卷：86
• 期：22
• 页码：11459-11463
• 全文大小：284K
• ISSN：1520-6882

文摘

Hydrogen sulfide (H₂S) has been regarded as the third important gaseous signaling molecule involved in human physiological and pathological processes. Due to the high reactive and diffusible properties of H₂S, real-time detection of H₂S fluctuations in living biological specimens is crucial. Here, we present a Cu(II)-metalated 3D porous nanoscale metal鈥搊rganic framework (nano-MOF) {CuL[AlOH]₂}_n (PAC; H₆L = meso-tetrakis(4-carboxylphenyl)porphyrin) and successfully employ this nano-MOF as a novel heterogeneous fluorescence probe for H₂S detection. As far as we know, nano-MOFs have never been used as selective fluorescence probes for H₂S detection. On the basis of the advantages of nano-MOF materials, this biocompatible nano-MOF probe exhibits rapid response, excellent selectivity, and hypotoxicity in in situ detection of H₂S and represents the most sensitive fluorescence probe for selective H₂S detection under physiological pH. In addition, confocal imaging was achieved successfully in living cells.