Activation of BNGR-A24 by Direct Interaction with Tachykinin-Related Peptides from the Silkworm Bombyx mori Leads to the Gq- and Gs-Coupled Signaling Cascades

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• 作者：Xiaobai He ; Jiashu Zang ; Xiangmei Li ; Jiajie Shao ; Huipeng Yang ; Jingwen Yang ; Haishan Huang ; Linjie Chen ; Liangen Shi ; Chenggang Zhu ; Guozheng Zhang ; Naiming Zhou
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：October 28, 2014
• 年：2014
• 卷：53
• 期：42
• 页码：6667-6678
• 全文大小：732K
• ISSN：1520-4995

文摘

Tachykinins constitute one of the largest peptide families in the animal kingdom and exert their diverse actions via G protein-coupled receptors (GPCRs). In this study, the Bombyx tachykinin-related peptides (TKRPs) were identified as specific endogenous ligands for the Bombyx neuropeptide GPCR A24 (BNGR-A24) and thus designated BNGR-A24 as BmTKRPR. Using both mammalian cell line HEK293 and insect cell line Sf21, further characterization demonstrated that BmTKRPR was activated, thus resulting in intracellular accumulation of cAMP, Ca²⁺ mobilization, and ERK1/2 phosphorylation in a G_s and G_q inhibitor-sensitive manner. Moreover, quantitative reverse transcriptase polymerase chain reaction analysis and dsRNA-mediated knockdown experiments suggested a possible role for BmTKRPR in the regulation of feeding and growth. Our findings enhance the understanding of the Bombyx TKRP system in the regulation of fundamental physiological processes.