New Tyrosinase Inhibitors from Paecilomyces gunnii

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• 作者：Ruili Lu ; Xiaoxiao Liu ; Sha Gao ; Wancun Zhang ; Fan Peng ; Fenglin Hu ; Bo Huang ; Longyun Chen ; Guanhu Bao ; Chunru Li ; Zengzhi Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2014
• 出版时间：December 10, 2014
• 年：2014
• 卷：62
• 期：49
• 页码：11917-11923
• 全文大小：279K
• ISSN：1520-5118

文摘

Through screening 50 strains of entomopathogenic fungi and rescreening of 7 strains of Paecilomyces gunnii, a methanol extract of liquid-cultivated mycelia of P. gunnii was found to have the strongest tyrosinase inhibitory activity. Preparative high-speed counter-current chromatography (HSCCC) guided by high-performance liquid chromatography (HPLC)鈥揺lectrospray ionization (ESI)鈥揾igh-resolution mass spectrometry (HRMS) was employed for the isolation and purification of the active components, and three new compounds with half inhibition concentration (IC₅₀) of 0.11, 0.17, and 0.14 mM against diphenolase were obtained from the extract, respectively. Their chemical structures were identified by HRMS, one- and two-dimensional nuclear magnetic resonance (2D NMR) spectroscopy as paecilomycones A, B, and C. Structure and activity studies showed that the tyrosinase inhibition activities are positively related to the number of hydroxyl groups on the paecilomycones.

Keywords:

Paecilomyces gunnii; phenalenones; tyrosinase inhibition