Characterization and NO Inhibitory Activities of Chemical Constituents from an Edible Plant Petasites tatewakianus

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• 作者：Meicheng Wang ; Qiang Zhang ; Hao Wang ; Quanhui Ren ; Yihang Sun ; Chunfeng Xie ; Jing Xu ; Da-Qing Jin ; Yasushi Ohizumi ; Yuanqiang Guo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2014
• 出版时间：September 24, 2014
• 年：2014
• 卷：62
• 期：38
• 页码：9362-9367
• 全文大小：270K
• ISSN：1520-5118

文摘

Petasites tatewakianus is an edible plant belonging to the family Compositae. In our continuous search for NO inhibitors, which may be useful for the development of anti-inflammatory agents, the chemical constituents of the leaves of the edible plant P. tatewakianus were investigated. This phytochemical investigation led to the isolation of 3 new (1鈥?b>3) and 10 known (4鈥?b>13) sesquiterpenes and 2 other types of known compounds (14 and 15). Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analyses, and the absolute configurations of compounds 1 and 3 were confirmed by comparing their experimental CD spectra with those calculated by the time-dependent density functional theory (TDDFT) method. The following biological studies disclosed that these isolated compounds showed inhibitory activities on LPS-induced NO production in murine microglial BV-2 cells. The results of our phytochemical investigation, including two new bakkenolide sesquiterpenes (1 and 2), one new sesquiterpene with an unusual carbon skeleton (3), and the first report of compounds 5鈥?b>7 and 10鈥?b>15 from this species, further revealed the chemical composition of P. tatewakianus as an edible plant, and the biological studies implied that P. tatewakianus, containing bioactive substances with the inhibitory activities of NO production, was potentially beneficial to human health.

Keywords:

edible plant; Petasites tatewakianus; sesquiterpenes; NO inhibitory activities; TDDFT CD calculations