An 鈥淓nhanced PET鈥?Based Fluorescent Probe with Ultrasensitivity for Imaging Basal and Elesclomol-Induced HClO in Cancer Cells

详细信息    查看全文

• 作者：Hao Zhu ; Jiangli Fan ; Jingyun Wang ; Huiying Mu ; Xiaojun Peng
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：September 17, 2014
• 年：2014
• 卷：136
• 期：37
• 页码：12820-12823
• 全文大小：283K
• ISSN：1520-5126

文摘

Reactive oxygen species (ROS) and cellular oxidant stress have long been associated with cancer. Unfortunately, the role of HClO in tumor biology is much less clear than for other ROS. Herein, we report a BODIPY-based HClO probe (BClO) with ultrasensitivity, fast response (within 1 s), and high selectivity, in which the pyrrole group at the meso position has an 鈥渆nhanced PET鈥?effect on the BODIPY fluorophore. The detection limit is as low as 0.56 nM, which is the highest sensitivity achieved to date. BClO can be facilely synthesized by a Michael addition reaction of acryloyl chloride with 2,4-dimethylpyrrole and applied to image the basal HClO in cancer cells for the first time and the time-dependent HClO generation in MCF-7 cells stimulated by elesclomol, an effective experimental ROS-generating anticancer agent.