Quantitative Proteomic Profiling Reveals Differentially Regulated Proteins in Cystic Fibrosis Cells

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• 作者：Navin Rauniyar ; Vijay Gupta ; William E. Balch ; John R. Yates ; III
• 刊名：Journal of Proteome Research
• 出版年：2014
• 出版时间：November 7, 2014
• 年：2014
• 卷：13
• 期：11
• 页码：4668-4675
• 全文大小：356K
• ISSN：1535-3907

文摘

The most prevalent cause of cystic fibrosis (CF) is the deletion of a phenylalanine residue at position 508 in CFTR (螖F508-CFTR) protein. The mutated protein fails to fold properly, is retained in the endoplasmic reticulum via the action of molecular chaperones, and is tagged for degradation. In this study, the differences in protein expression levels in CF cell models were assessed using a systems biology approach aided by the sensitivity of MudPIT proteomics. Analysis of the differential proteome modulation without a priori hypotheses has the potential to identify markers that have not yet been documented. These may also serve as the basis for developing new diagnostic and treatment modalities for CF. Several novel differentially expressed proteins observed in our study are likely to play important roles in the pathogenesis of CF and may serve as a useful resource for the CF scientific community.

Keywords:

Cystic Fibrosis; cystic fibrosis transmembrane conductance regulator; CFTR; MudPIT; mass spectrometry; NSAF; spectral count; label free; proteomics