Optimization of an Immobilized-Cell Biocatalyst for Production of 4-Cyanopentanoic Acid

详细信息    查看全文

• 作者：Eugenia C. Hann ; Amy E. Sigmund ; Susan M. Hennessey ; John E. Gavagan ; David R. Short ; Arie Ben-Bassat ; Sarita Chauhan ; Robert D. Fallon ; Mark S. Payne ; and Robert DiCosimo
• 刊名：Organic Process Research & Development
• 出版年：2002
• 出版时间：July 2002
• 年：2002
• 卷：6
• 期：4
• 页码：492 - 496
• 全文大小：52K
• 年卷期：v.6,no.4(July 2002)
• ISSN：1520-586X

文摘

Optimization of microbial cell immobilization, catalyst specificactivity, and volumetric productivity were required for scale-up of the nitrilase-catalyzed hydrolysis of 2-methylglutaronitrileto 4-cyanopentanoic acid, an intermediate in the preparationof 1,5-dimethyl-2-piperidone. As an alternative to the immobilization of Acidovorax facilis 72W cells in carrageenan,immobilization in alginate, followed by cross-linking withglutaraldehyde and polyethylenimine, produced a catalyst whichwas stable in reaction mixtures containing high concentrationsof 4-cyanopentanoic acid ammonium salt. Immobilization inalginate produced catalysts with a higher nitrilase specificactivity than was achieved in carrageenan, and volumetricproductivity of 4-cyanopentanoic acid was increased from 19to 49 g/L/h. Substituting alginate for carrageenan also eliminated one process step in the immobilization. A further increasein volumetric productivity to 79 g/L/h was achieved by usingan immobilized Escherichia coli transformant which expressesA. facilis 72W nitrilase.