A Photostable Near-Infrared Fluorescent Tracker with pH-Independent Specificity to Lysosomes for Long Time and Multicolor Imaging

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• 作者：Xinfu Zhang ; Chao Wang ; Zhuo Han ; Yi Xiao
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：December 10, 2014
• 年：2014
• 卷：6
• 期：23
• 页码：21669-21676
• 全文大小：512K
• ISSN：1944-8252

文摘

A new boron鈥揹ipyrromethene-based lysosome tracker, Lyso-NIR, is facilely synthesized. Besides the intensive near-infrared (NIR) fluorescence and high photostability, Lyso-NIR shows the capability to stably localize in lysosomes, which is independent of the local pH. Lyso-NIR does not have the problematic alkalization effect suffered by the commonly used lysotrackers; thus, it shows ignorable cytotoxicity and slightly affects normal physiological functions of lysosomes. The above advantages of Lyso-NIR make it feasible to track lysosomes鈥?dynamic changes in a relatively long time during the full cellular processes such as apoptosis, heavy metal stimulation, and endocytosis, as is demonstrated in this work. Moreover, Lyso-NIR鈥檚 narrow NIR emission at 740 nm with a full width at half-maximum smaller than 50 nm makes it easy to avoid the crosstalk with the emissions from other common fluorescent probes, which strengthens Lyso-NIR鈥檚 competitiveness as a standard lysosome tracker for multicolor bioimaging.

Keywords:

lysosome; probe; near-infrared; fluorescence imaging