Enhanced Magnetic Fluid Hyperthermia by Micellar Magnetic Nanoclusters Composed of MnxZn1鈥?i>xFe2O4 Nanoparticles for Induced Tumor Cell Apoptosi

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• 作者：Yang Qu ; Jianbo Li ; Jie Ren ; Junzhao Leng ; Chao Lin ; Donglu Shi
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：October 8, 2014
• 年：2014
• 卷：6
• 期：19
• 页码：16867-16879
• 全文大小：792K
• ISSN：1944-8252

文摘

Monodispersed Mn_xZn_1鈥?i>xFe₂O₄ magnetic nanoparticles of 8 nm are synthesized and encapsulated in amphiphilic block copolymer for development of the hydrophilic magnetic nanoclusters (MNCs). These MNCs exhibit superparamagnetic characteristics, high specific absorption rate (SAR), large saturation magnetization (M_s), excellent stability, and good biocompatibility. MnFe₂O₄ and Mn_0.6Zn_0.4Fe₂O₄ are selected as optimum compositions for the MNCs (MnFe₂O₄/MNC and Mn_0.6Zn_0.4Fe₂O₄/MNC) and employed for magnetic fluid hyperthermia (MFH) in vitro. To ensure biosafety of MFH, the parameters of alternating magnetic field (AMF) and exposure time are optimized with low frequency, f, and strength of applied magnetic field, H_applied. Under optimized conditions, MFH of MnFe₂O₄/MNC and Mn_0.6Zn_0.4Fe₂O₄/MNC result in cancer cell death rate up to 90% within 15 min. The pathway of cancer cell death is identified as apoptosis, which occurs in mild hyperthermia near 43 掳C. Both MnFe₂O₄/MNC and Mn_0.6Zn_0.4Fe₂O₄/MNC show similar efficiencies on drug-sensitive and drug-resistant cancer cells. On the basis of these findings, those Mn_xZn_1鈥?i>xFe₂O₄ nanoclusters can serve as a promising candidate for effective targeting, diagnosis, and therapy of cancers. The multimodal cancer treatment is also possible as amphiphilic block copolymer can encapsulate, in a similar fashion, different nanoparticles, hydrophobic drugs, and other functional molecules.

Keywords:

magnetic nanoclusters; self-assembly; magnetic fluid hyperthermia; specific absorption rate; cell apoptosis