Perylene-cored Star-shaped Polycations for Fluorescent Gene Vectors and Bioimaging

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• 作者：Shusen You ; Qing Cai ; Yang Zheng ; Bicheng He ; Jie Shen ; Wantai Yang ; Meizhen Yin
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：September 24, 2014
• 年：2014
• 卷：6
• 期：18
• 页码：16327-16334
• 全文大小：458K
• ISSN：1944-8252

文摘

Two star polycations, poly(2-aminoethyl methacrylate) (PAEMA, P1) and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA, P2), have been synthesized with perylene diimide (PDI) as the central fluorophore. ¹H NMR and ¹³C NMR are used to confirm the successful synthesis of a macromolecular initiator. Using ATRP strategy, P1 and P2 are obtained with narrow molecular weight distribution. The star polymers have good fluorescence properties in aqueous solution, which provides fluorescent tracing and imaging during gene delivery. Both P1 and P2 can efficiently condense DNA into stable nanoparticles. Transfection studies demonstrate that P1 and P2 deliver DNA into live cells with higher efficiency and lower cytotoxicity than polyethylenimine (PEI, 25 kDa). P2 shows higher capacity for gene delivery than P1 due to its better buffering and faster rate of cellular internalization.

Keywords:

star polymer; gene vector; fluorescence; bioimaging