Design, Construction, and Characterization of a Set of Biosensors for Aromatic Compounds

详细信息    查看全文

• 作者：Haoran Xue ; Hailing Shi ; Zhou Yu ; Shuaixin He ; Shiyu Liu ; Yuhang Hou ; Xingjie Pan ; Huan Wang ; Pu Zheng ; Can Cui ; Helena Viets ; Jing Liang ; Yihao Zhang ; Shuobing Chen ; Haoqian M. Zhang ; Qi Ouyang
• 刊名：ACS Synthetic Biology
• 出版年：2014
• 出版时间：December 19, 2014
• 年：2014
• 卷：3
• 期：12
• 页码：1011-1014
• 全文大小：342K
• ISSN：2161-5063

文摘

Aromatic pollutants in the environments pose significant threat to human health due to their persistence and toxicity. Here, we report the design and comprehensive characterization of a set of aromatic biosensors constructed using green fluorescence protein as the reporter and aromatics-responsive transcriptional regulators, namely, NahR, XylS, HbpR, and DmpR, as the detectors. The genetic connections between the detectors and the reporter were carefully adjusted to achieve fold inductions far exceeding those reported in previous studies. For each biosensor, the functional characteristics including the dose鈥搑esponses, dynamic range, and the detection spectrum of aromatic species were thoroughly measured. In particular, the interferences that nontypical inducers exert on each biosensor鈥檚 response to its strongest inducer were evaluated. These well-characterized biosensors might serve as potent tools for environmental monitoring as well as quantitative gene regulation.