Hairpin DNA-Assisted Silicon/Silver-Based Surface-Enhanced Raman Scattering Sensing Platform for Ultrahighly Sensitive and Specific Discrimination of Deafness Mutations in a Real System

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• 作者：Hui Wang ; Xiangxu Jiang ; Xing Wang ; Xinpan Wei ; Ying Zhu ; Bin Sun ; Yuanyuan Su ; Sudan He ; Yao He
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：August 5, 2014
• 年：2014
• 卷：86
• 期：15
• 页码：7368-7376
• 全文大小：651K
• ISSN：1520-6882

文摘

Surface-enhanced Raman scattering (SERS) is well-recognized as a powerful analytical tool for ultrahighly sensitive detection of analytes. In this article, we present a kind of silicon-based SERS sensing platform made of a hairpin DNA-modified silver nanoparticles decorated silicon wafer (AgNPs@Si). In particular, the AgNPs@Si with a high enhancement factor (EF) value of 4.5 脳 10⁷ is first achieved under optimum reaction conditions (i.e., pH = 12, reaction time = 20 min) based on systematic investigation. Such resultant AgNPs@Si is then employed for construction of a silicon-based SERS sensing platform through surface modification of hairpin DNA, which is superbly suitable for highly reproducible, multiplexed, and ultrasensitive DNA detection. A detection limit of 1 fM is readily achieved in a very reproducible manner along with high specificity. Most significantly, for the first time, we demonstrate that the silicon-based SERS platform is highly efficacious for discriminating deafness-causing mutations in a real system at the femtomolar level (500 fM), which is about 3鈥? orders of magnitude lower than that (5 nM) ever reported by conventional detection methods. Our results raise the exciting potential of practical SERS applications in biology and biomedicine.