A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays

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• 作者：Yilei Yang ; Qinghao Zhang ; Mei Gao ; Xiaohong Yang ; Ziwei Huang ; Jing An
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：August 5, 2014
• 年：2014
• 卷：53
• 期：30
• 页码：4881-4883
• 全文大小：189K
• ISSN：1520-4995

文摘

We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.