Highly Sensitive Method for Specific, Brief, and Economical Detection of Glycoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by the Synthesis of a New Hydrazide Derivative

详细信息    查看全文

• 作者：Weitao Cong ; Ayi Zhou ; Zhiguo Liu ; Jiayi Shen ; Xuan Zhou ; Weijian Ye ; Zhongxin Zhu ; Xinliang Zhu ; Jianjun Lin ; Litai Jin
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 3, 2015
• 年：2015
• 卷：87
• 期：3
• 页码：1462-1465
• 全文大小：299K
• ISSN：1520-6882

文摘

A new hydrazide derivative was synthesized and used for the first time as a specific, brief, and economical probe to selectively visualize glycoproteins in 1-D and 2-D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with high sensitivity. The detection limit of the newly developed staining method is 2- and 4-fold higher than that of the widely used Pro-Q Emerald 300 and 488 stains, respectively.