High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl 尾-D-Maltoside

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• 作者：Jing Liu ; Fangjun Wang ; Jiawei Mao ; Zhang Zhang ; Zheyi Liu ; Guang Huang ; Kai Cheng ; Hanfa Zou
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 17, 2015
• 年：2015
• 卷：87
• 期：4
• 页码：2054-2057
• 全文大小：248K
• ISSN：1520-6882

文摘

N-dodecyl 尾-D-maltoside (DDM), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for N-glycoproteomic analysis of minute protein sample from mouse brain tissue. After combining with the capillary-based glycoproteomic reactor, 281 N-glycosylation sites were successfully characterized from 50 渭g of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies.