Complete Perchlorate Reduction Using Methane as the Sole Electron Donor and Carbon Source
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- 作者：Yi-Hao Luo ; Ran Chen ; Li-Lian Wen ; Fan Meng ; Yin Zhang ; Chun-Yu Lai ; Bruce E. Rittmann ; He-Ping Zhao ; Ping Zheng
- 刊名：Environmental Science & Technology
- 出版年：2015
- 出版时间：February 17, 2015
- 年：2015
- 卷：49
- 期：4
- 页码：2341-2349
- 全文大小：416K
- ISSN：1520-5851
文摘
Using a CH4-based membrane biofilm reactor (MBfR), we studied perchlorate (ClO4鈥?/sup>) reduction by a biofilm performing anaerobic methane oxidation coupled to denitrification (ANMO-D). We focused on the effects of nitrate (NO3鈥?/sup>) and nitrite (NO2鈥?/sup>) surface loadings on ClO4鈥?/sup> reduction and on the biofilm community鈥檚 mechanism for ClO4鈥?/sup> reduction. The ANMO-D biofilm reduced up to 5 mg/L of ClO4鈥?/sup> to a nondetectable level using CH4 as the only electron donor and carbon source when CH4 delivery was not limiting; NO3鈥?/sup> was completely reduced as well when its surface loading was 鈮?.32 g N/m2-d. When CH4 delivery was limiting, NO3鈥?/sup> inhibited ClO4鈥?/sup> reduction by competing for the scarce electron donor. NO2鈥?/sup> inhibited ClO4鈥?/sup> reduction when its surface loading was 鈮?.10 g N/m2-d, probably because of cellular toxicity. Although Archaea were present through all stages, Bacteria dominated the ClO4鈥?/sup>-reducing ANMO-D biofilm, and gene copies of the particulate methane mono-oxygenase (pMMO) correlated to the increase of respiratory gene copies. These pieces of evidence support that ClO4鈥?/sup> reduction by the MBfR biofilm involved chlorite (ClO2鈥?/sup>) dismutation to generate the O2 needed as a cosubstrate for the mono-oxygenation of CH4.
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