Electrostatic Trapping of Double-Stranded DNA Based on Cd(OH)2 Three-Side Nanobelt Architectures

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• 作者：Di Bao ; Peng Gao ; Yanbo Wang ; Han Zhou ; Yujin Chen ; Guorui Chen ; Piaoping Yang ; Xitian Zhang
• 刊名：Journal of Physical Chemistry C
• 出版年：2015
• 出版时间：January 29, 2015
• 年：2015
• 卷：119
• 期：4
• 页码：1953-1959
• 全文大小：462K
• ISSN：1932-7455

文摘

In this paper, Cd(OH)₂ 1D three-side nanobelt architectures have been fabricated through a facile two-phase reaction system. Considering their electropositive character proven by zeta potential measurement, the as-obtained Cd(OH)₂ nanomaterials have been applied to capture negatively charged DNA. On the basis of the above results, the Cd(OH)₂ nanomaterials have been employed for trapping negatively charged double-stranded DNA molecules (salmon sperm DNA). Assisted by the detailed observation of their DNA interaction results as well as through UV鈥搗is spectroscopy, infrared imaging automatic target recognition (IRATR) and inductive coupled plasma atomic emission spectrometer (ICP-AES) measurements, it has been clearly shown that this 1D nanoarchitecture can effectively trap and release DNA molecules without use of any other organic reagents. In addition, a miniaturized electrochemical DNA-releasing device based on the Cd(OH)₂ 1D three-side nanobelt architectures has been fabricated. Using this device, it is possible to recycle the Cd(OH)₂ nanostructures. In addition, the Cd(OH)₂ nanomaterials maintain their nanostructures, and no Cd²⁺ ions were released in repeated DNA separation and transfer processes, which demonstrate their practicality for DNA trapping.