X-DNA Origami-Networked Core-Supported Lipid Stratum

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• 作者：Seung Won Shin ; Kyung Soo Park ; Min Su Jang ; Woo Chul Song ; Jin Kim ; Seung-Woo Cho ; Joo Young Lee ; Jeong Ho Cho ; Sunghwan Jung ; Soong Ho Um
• 刊名：Langmuir
• 出版年：2015
• 出版时间：January 27, 2015
• 年：2015
• 卷：31
• 期：3
• 页码：912-916
• 全文大小：343K
• ISSN：1520-5827

文摘

DNA hydrogels are promising materials for various fields of research, such as in vitro protein production, drug carrier systems, and cell transplantation. For effective application and further utilization of DNA hydrogels, highly effective methods of nano- and microscale DNA hydrogel fabrication are needed. In this respect, the fundamental advantages of a core鈥搒hell structure can provide a simple remedy. An isolated reaction chamber and massive production platform can be provided by a core鈥搒hell structure, and lipids are one of the best shell precursor candidates because of their intrinsic biocompatibility and potential for easy modification. Here, we demonstrate a novel core鈥搒hell nanostructure made of gene-knitted X-shaped DNA (X-DNA) origami-networked gel core-supported lipid strata. It was simply organized by cross-linking DNA molecules via T4 enzymatic ligation and enclosing them in lipid strata. As a condensed core structure, the DNA gel shows Brownian behavior in a confined area. It has been speculated that they could, in the future, be utilized for in vitro protein synthesis, gene-integration transporters, and even new molecular bottom-up biological machineries.